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KMID : 0370219950390060676
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Yakhak Hoeji
1995 Volume.39 No. 6 p.676 ~ p.680
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Nucleotide Sequences of Rep and CAT Proteins encoded by Chloramphenicol-Resistance Plasmid pKH7
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윤성준/Yoon SJ
이대운/김우구/신철교/임성환/문경호/Lee DW/Kim WK/Shin CK/Im SH/Moon KH
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Abstract
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The nucleotide sequence of XbaI-MboI fragment of pKH7, a chloramphenicol-resistant(Cmr) plasmid isolated from multidrug-resistant S. aureus SA2, has been determined. XbaI-MboI fragment of pKH7 was found to contain two ORFs. One ORF encoded Rep and the other encoded CAT protein. The deduced amino acid sequences of Rep and CAT of pKH7 were compared to those of pUB112 and pC221. Comparisons revealed that there was one amino acid dfference in CAT between pKH7 and pUB112. CAT of pKH7 exhibited 98.6% amino acid identity to that of pC221. In case of Rep proteins, a slightly lower homology of 96.4% and 86.7% in amino acid sequences was observed betweeen pKH7 and pUB112 and between pKH7 and pC221, respectively.
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KEYWORD
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